녹아웃(KO) 마우스 | Cyagen Korea

사육 서비스

Mouse and Rat Breeding Services

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◆ Transgenic Founder Mice/Rats Breeding to F1

For most transgenic projects, the deliverables are F0 founder animals. Given that the transgenes are randomly integrated into genome, and a small percentage of founder animals display some degree of mosaicism, there is a possibility that some F0 animals do not transmit the transgene to their offspring. Cyagen will breed founder animals to wt animals of matching background, and screen F1 animals with desired mutation(s). A minimum of 2 F1 animals per line will be generated and delivered.

 

Service Price Turnaround Time
Transgenic founder mice/rats breeding to F1 mice $1,450/first line, $1,000/per additional line ~3 months
rats $1,950/first line, $1,200/per additional line ~3 months

Note: Large cohorts of F1 or F2 animals can be generated at additional cost.

◆ ES Cell Gene Targeted Heterozygous Mice Breeding to Homozygous Mice

1. Standard KO/KI mice breeding service

Cyagen will set up heterozygote x heterozygote breeding and screen pups by PCR for targeted mutation(s). Theoretically this breeding will produce 50% heterozygotes, 25% homozygotes and 25% wt animals. We will provide 2~4 homozygous mice per line. If no homozygotes are identified from a total of 30 pups screened, it is possible that the homozygous mutation may lead to embryonic lethality. If this is the case we will stop breeding and discuss the project with clients.

 

2. Conditional KO/KI mice breeding service

Cyagen can inter-cross flox/wt mice to generate flox/flox mice, and can further breed them with Cre deleter of your choice to produce tissue-specific KO/KO (or KI/KI) mice. We will deliver 2~4 flox/flox mice or 2~4 tissue-specific KO/KO (or KI/KI) mice.

 

3. Price and turnaround time

Service Price Turnaround Time
Standard KO/KI mice breeding service $3,450 3~6 months
Conditional KO/KI mice breeding service flox/flox $3,450 3~6 months
tissue-specific KO/KO and KI/KI $6,450 5~9 months

Note: Large cohorts of homozygous animals can be generated at additional cost.

◆ CRISPR-mediated Genome Editing Mice/Rats Breeding

Although animals generated by CRISPR-mediated genome editing carry targeted mutation(s), the founder animals are different from animals generated by ES cell gene targeting approaches in that most of the founder animals display some degree of mosaicism. Cyagen offers founder animal breeding service which allows us to deliver germline transmission-confirmed heterozygous/homozygous animals to researchers.

 

We guarantee generation and delivery of a minimum of 3 heterozygotes. We can further inter-cross heterozygotes and deliver homozygous animals (a minimum of 2 per line). If no homozygotes are identified from a total of 30 pups screened, it is possible that the homozygous mutation may lead to embryonic lethality. If this is the case we will stop breeding and discuss the project with clients.

Service Price  Turnaround Time        
Mice Rats

CRISPR-mediated genome editing mice/rats breeding 

heterozygous

$1,450/first line, $1,000/per additional line

$1,950/first line, $1,200/per additional line  

3~4 months
homozygous

$2,450/first line, $1,500/per additional line

$3,450/first line, $2,000/per additional line

4~8 months

Note: Large cohorts of homozygous animals can be generated at additional cost.

◆ Colony Maintenances Service
  • For most projects we terminate the animal colony in our facility after successful delivery of mutant animals to the client’s facility. We can maintain small colonies of mutant mouse/rat line(s) upon client’s request. The colonies will be maintained as heterozygotes/wt animal breeding pairs.
  • Price: $200/line/month (mice), $300/line/month (rats)
  • In addition to breeding services, Cyagen can back up your live animal colony by our embryo and sperm cryopreservation services, which can not only protect your valuable mutation strains but also reduce your cost in the long run. 

Cyagen을 선택하는 이유?

  • 15년 이상의 맞춤형 동물 모델 제작 경험이 있습니다.
  • 78,000 마리 이상의 Mouse 및 Rat 모델을 전 세계로 제공해 왔습니다.
  • SCI 저널에4,700 건이 넘는 인용문이 실렸습니다.
  • 전 세계 3,000개 이상의 생물 과학 업체와 협력 관계를 맺고 있습니다.
  • 대부분의 서비스에 100% 환불 보장 옵션을 제공합니다.
  • AAALAC 인증 및 OLAW 보장
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