Cancer cells, hard-to-transfect cells, stem cells and more.
Our CRISPR-Pro technology can bring paired gRNAs by which large fragment deletion can be obtained. Compared with frameshift mutations, the removal of large fragments of the gene is more efficient and straight-forward.
| Comparison |
Large fragment KO |
Frameshift mutation | ||
| Plasmid electroporation |
Lentivirus transduction |
Plasmid electroporation | Lentivirus transduction | |
| Outcomes |
Complete deletion of large fragment enables inactivation for all transcripts, much likely including unknown transcripts as well. |
Transcripts from downstream and some unknown transcripts may not be affected. | ||
| Deletion Limit |
Large fragments |
One or several base pairs | ||
| Identification |
Easy by PCR assays |
Sequencing needed |
||
| If Virus Packaging Needed | No |
Yes |
No | Yes |
| Turnaround | 2~3 months |
3~5 months |
2~3 months | 3~5 months |
Request a quote now. Alternatively, you can always email service-apac@cyagen.com to inquire about our services or obtain a quote for your project.