Cancer cells, hard-to-transfect cells, stem cells and more.
Our CRISPR-Pro technology can bring paired gRNAs by which large fragment deletion can be obtained. Compared with frameshift mutations, the removal of large fragments of the gene is more efficient and straight-forward.
Large fragment KO
|Plasmid electroporation||Lentivirus transduction|
Complete deletion of large fragment enables inactivation for all transcripts, much likely including unknown transcripts as well.
|Transcripts from downstream and some unknown transcripts may not be affected.|
|One or several base pairs|
Easy by PCR assays
|If Virus Packaging Needed||No||
|2~3 months||3~5 months|
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