Cyagen의 전문가가 프로젝트 요구 사항에 대한
견적,
정보 및 예상 소요시간을 제공해 드릴 것
입니다.
"Among several commercial services who have claimed to be specialized to generate mouse models with genetic modifications, I recognize that Cyagen is one of the best. We are looking forward to having more cooperation with them."
Korea Brain Research Institute (KBRI)영업일 기준 1-2일 내에 답변해 드리겠습니다.
Catalog Number: I001211
Strain Name: C57BL/6JCya-Gt(ROSA)26Sorem1(CAG-KikGR)/Cya
Genetic Background: C57BL/6JCya
Strain 설명
This strain is a fluorescently labeled model carrying a photoconvertible green-to-red fluorescent protein (KikGR) reporter gene expression element, with the expression of KikGR protein regulated by the CAG promoter. KikGR fluorescent protein is suitable for cell labeling and lineage tracing. KikGR protein’s photoconversion is instant and efficient, and it shows almost no toxicity when highly expressed in ES cells, making KikGR the fluorescent protein of choice for use in ES cells and mouse embryos [1]. The CAG promoter is constructed by integrating the chicken β-actin gene promoter, the cytomegalovirus (CMV) early enhancer, the rabbit β-actin gene splice acceptor, and the bovine globin polyA signal, known for its strong and stable real-time gene expression in various mammalian cells, making it a popular choice for driving gene expression in transgenic models.
Rosa26-CAG-KikGR mice are constructed by integrating the CAG promoter-Kozak-KikGR-rBG pA gene expression cassette into intron 1 of the mouse Rosa26 safe harbor locus using gene editing technology. KikGR protein is systemically expressed in the mice.
Strain 유전자 편집 전략
Figure 1. Gene editing strategy of Rosa26-CAG-KikGR mice. The CAG promoter-Kozak-KikGR-rBG pA cassette was cloned into the intron 1 of ROSA26.
참고문헌