Introduction: Heart failure is a syndrome caused by myocardial damage, which results from myocardial infarction, cardiomyopathy and inflammation, etc. Such myocardial damage leads to changes in myocardial structure and function, resulting in a low capability of ventricular filling and pumping. Chronic Heart Failure (CHF) refers to a long-term and consistent state of heart failure, which can be classified as stable, compensated, or decompensated. In general, the surgical models are achieved by either permanent ligation of left coronary artery, cardiac ischemia-reperfusion injury, or Transverse Aortic Constriction (TAC). It usually takes a few days or several weeks to develop into consistent and end-stage heart failure. This model can be used in basic medicine or new drugs preclinical research.
Animal requirement: Rats and mice - males are preferable. In special cases, female animals may be used. Age and weight can be adjusted accordingly.
CHF can be achieved by three surgical models as below:
- 1) Myocardial infarction induced by permanent ligation of the left coronary artery;
- 2) Heart failure induced by cardiac ischemia-reperfusion injury;
- 3) Heart failure induced by Transverse Aortic Constriction (TAC)
Chronic experiment. Postoperative time ranges from a few days to several weeks.
Pre/Post-operative management and delivery:
The whole process, including the breeding, surgery and post-operative management is carried out in Cyagen AAALAC-accredited SPF-level breeding facility.
Before the surgery, animals can be supplied by customers. Alternatively, Cyagen can help purchase Bio-standard animals (C57 mice, Wistar rats, or any commercially available GEMs worldwide). After full recovery from surgery, disease models can be delivered to customers by dedicated express.
- After-surgery routine observation: daily activities and diet intake are observed by full-time veterinarians, and pain relief treatment is applied accordingly.
- Cardiac ultrasonography: comprehensive evaluation of cardiac structure and function is achieved by ultra-high-definition small-animal-specific ultrasound machine. The evaluation of cardiac function before and after surgery (different periods of ventricular hypertrophy or heart failure development) includes the wall thickness (IVSs, IVSd, LVPWs, LVPWd), ventricular volume (LV Vols, LV Vold), ventricular lumen diameter (LVIDs, LVIDd), ejection fraction (EF%), fractional shortening (FS%) etc.. Customized ultrasonic tests can be performed upon request.
- Post-operative electrocardiogram detection: ECG records analysis at multiple time points.
- Blood sample collection: 0.7-1.0 mL venous blood, collected from adult mice or rats according to weight, can be used for various biochemical tests. The serum or plasma is stored in a -80⁰C refrigerator until delivery or biochemical detection.
- Cardiopulmonary specimen collection: Cardiopulmonary specimens are collected from different sections. The heart and lung specimens can be used to calculate the heart weight to body weight ratio and lung weight to body weight ratio. Besides, the specimens can be stored in a -80⁰C refrigerator or fixed with formalin for further pathological examination.
Animal ethical censorship and management
The Institutional Animal Care and Use Committee (IACUC) reviews the experimental protocol and manages the entire surgical procedure, including anesthesia, analgesia, animal preparation, and post-operative management.
For any surgical disease model, the strategy can be adjusted according to the scientific problems that researchers want to solve. If you have any special requests, please contact 86 20-31601779 or email firstname.lastname@example.org. Cyagen provides surgical model services personalized for your research goals - our senior technical team will develop a detailed experimental plan according to your needs and keep you updated throughout the model generation process.
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