Highlights:
- No animal-derived components, thus reducing the chance of contamination.
- Comparable performance in dissociating cells from the plasticware to trypin.
- Can be added directly into the cell culture system for digestion without the need to wash cells after aspirating off spent medium.
- Inhibitors are not needed to end digestion.
- Batch-to-batch consistency.
Stability/Storage:
- Store at -20oC for up to one year and 2-8oC for up to one month.
- For optimal performance, avoid repeated warm-cooling and freeze-thawing.
Quality Control:
Standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
3. Osmolality
4. Endotoxin