CRISPR/Cas9 and TurboKnockout-Related Seminar 10: Mitchell Cancer Institute University of South Alabama
Date: May 17, 2016
While genetically engineered mouse/rat models have been used widely in biomedical research, the design and generation of these models can be complicated, time-consuming and costly. In this presentation, Dr. Ouyang will briefly review the traditional pronuclear injection based transgenics and ES cell homologous recombination based gene targeting, and go over the recently developed CRISPR-mediated genome editing technology, with an emphasis on the pros and cons of each technology and their applications. He will then introduce TurboKnockout®, the newest ES-cell based gene targeting technology which allows the generation of conditional knockout/knockin mouse models in as fast as 6 months, 4-6 months shorter than the standard approach. Lastly, Dr. Ouyang will highlight a novel online tool called VectorBuilder, which promises to revolutionize the way DNA vector cloning is done.
Time: Tuesday, May 17, 2016
3:00 pm – 4:00 pm
Location: Multi-purpose Room | Mitchell Cancer Institute University of South Alabama
1660 Springhill Avenue