Cre/lox 시스템은 유전자의 특이적 및 시간적 발현에 대한 높은 수준의 제어로 tissue-specific 또는 inducible knockout의 생성을 가능하게 한다.
CRISPR Capabilities for Rat Models:
● Standard Use - Generate tissue-specific or inducible knockouts, such as in the KO-first conditional allele strategy
a. Transgene Activation: A loxP-flanked “stop” sequence between the promoter and the coding sequence blocks expression (until bred with a Cre strain)
b. Inducible Knockout: A loxP-flanked transgene sequence may be removed upon breeding with a Cre strain
● Reporter Strains - Visually confirm locations of Cre recombinase activity with loxP-flanked fluorescent protein sequences