“Among several commercial services who have claimed to be specialized to generate mouse models with genetic modifications, I recognize that Cyagen is one of the best. We are looking forward to having more cooperation with them.”Korea Brain Research Institute (KBRI)
영업일 기준 1-2일 내에 답변해 드리겠습니다.
Strain Background: C57BL/6N
The humanized VISTA mouse model (hVISTA) is developed by Knockin at the mouse VISTA locus, and expresses a chimeric VISTA with a human extracellular, human transmembrane and murine intracellular domain.
Research and Application:
Figure 1. hVISTA expression pattern in hVISTA mice recapitulates mVISTA in WT mice. hVISTA and mVISTA expression on freshly isolated splenocytes on (A) Tregs (viable, CD3+CD4+CD25+FoxP3+), (B) CD11bhigh (viable, CD3-, CD19-), (C) conventional CD4+ (viable, CD3+CD4+FoxP3- ), and (D) CD8+ (viable, CD3+CD8+) T cells. VISTA is not detected on cells from VISTA KO homozygous mice.
Figure 2. hVISTA is functional on effector T cells. Splenic and LN-isolated T cells (CD4+CD25-) activated with αCD3 and hVISTA-Fc, mVISTAFc or IgG-Fc for 3 days. (A) Cell proliferation determined by CFSE dilution. (B) IL-2 production measured by ELISA in culture supernatant. Results are expressed as mean±SD.
Figure 3. Anti-tumor effects in response to anti-human VISTA + anti-mouse PD-1 treatment. Mice of indicated genotype were inoculated with MC38 tumor cells s.c. and treated αKLH (control), αmPD-1, αhVISTA or αmPD-1 + αhVISTA (combination). Tumor volume measurement in (A) wild-type and (B) hVISTA KI homozygous mice at different time points. Results are expressed as mean±SEM. (C) Survival of treated hVISTA KI homozygous mice analyzed up to 50 days post MC38 tumor inoculation. In hVISTA mice, the combination of αmPD-1 and αhVISTA has the best anti-tumor effect.
1. Eltanbouly M A, Zhao Y, Nowak E C, et al. VISTA is a checkpoint regulator for naïve T cell quiescence and peripheral tolerance. Science, 2020, 367(6475).
2. Johnston R J, Su L J, Pinckney J, et al. VISTA is an acidic pH-selective ligand for PSGL-1. Nature, 2019, 574(7779): 565-570.