녹아웃(KO) 마우스 | Cyagen Korea

hPD-1/hVISTA Mouse


>> 견적의뢰 또는 서비스문의
Cyagen의 전문가가 프로젝트 요구 사항에 대한
견적, 정보 및 예상 소요시간을 제공해 드릴 것
입니다.

Strain Background: C57BL/6N

 

Construction Strategy:

The double-humanized PD-1 and VISTA mouse model (hPD-1/hVISTA) was generated by intercrossing hPD-1 and hVISTA mice. Both hPD-1 and hVISTA expressions are regulated by endogenous mouse promoters.

 

PD-1/VISTA Features:

  •   The PD-1 and VISTA extracellular domains are entirely humanized
  •   Physiological regulation and expression pattern of the human PD-1 and VISTA
  •   Preservation of the target-ligand interaction
  •   Fully functional mouse immune system
  •   Lack of expression of the murine target gene, thus avoiding cross-reactivity

 

Research and Application:

The hPD-1/hVISTA mouse enables the in vivo efficacy and safety assessment of compounds targeting the human immune checkpoint PD-1 and/or VISTA in fully immunocompetent mice.

Use our hPD-1/hVISTA mouse model together with cell line MC38-hPD-L1 as a syngeneic tumor model for testing the efficacy of combination therapies targeting human PD-1 and human PD-L1.

 

Validation Data:

Figure 1. hPD-1 expression pattern in hPD-1/hVISTA mice recapitulates mPD-1 in wild-type mice. hPD-1 and mPD-1 expression on αCD3/αCD28-activated splenocytes for (A) Tregs and (B) conventional CD4+, alongside both (C) hPD-1 expression and mPD-1 expression in CD8+ T cells across hPD-1/hVISTA mice and wild-type mice at the indicated timepoints.    

Figure 2. hVISTA expression pattern in hPD-1/hVISTA mice recapitulates mVISTA in wild-type mice. hVISTA and mVISTA expression on freshly isolated splenocytes on Tregs, conventional CD4+, and CD8+ T cells.

Figure 3. Anti-tumor effects in response to anti-human VISTA + anti-mouse PD-1 treatment. Mice of indicated genotype were inoculated with MC38 tumor cells s.c. and treated αKLH (control), αmPD-1, αhVISTA or αmPD-1 + αhVISTA (combination). In wild-type mice, mPD-1 antibody can inhibit tumor proliferation, while hVISTA antibody can inhibit tumor proliferation in hVISTA mice. The combined antibody therapy of hVISTA and mPD-1 can inhibit tumor growth in hVISTA mice more effectively than either therapy used alone.

Figure 4. In vivo anti-tumor activity in response to pembrolizumab treatment in hPD-1 mice. Implanting GL261 murine glioma cells into wildtype and hPD-1 mice intracranially to detect the effects of different treatments on the survival of mice. In wild-type mice, hPD-1 and mPD-1 antibodies have no significant effect on the survival of mice, while in hPD-1 mice, the treatment of hPD-1 monoclonal antibody pembrolizumab can inhibit tumor growth and prolong the life of mice.

Cyagen을 선택하는 이유?

  • 15년 이상의 맞춤형 동물 모델 제작 경험이 있습니다.
  • 78,000 마리 이상의 Mouse 및 Rat 모델을 전 세계로 제공해 왔습니다.
  • SCI 저널에4,700 건이 넘는 인용문이 실렸습니다.
  • 전 세계 3,000개 이상의 생물 과학 업체와 협력 관계를 맺고 있습니다.
  • 대부분의 서비스에 100% 환불 보장 옵션을 제공합니다.
  • AAALAC 인증 및 OLAW 보장
위에 제품이나 서비스에 관심이 있으시면 저희한테 연락 부탁드립니다.

Your First Name can not be empty

Your Last Name can not be empty

Institution/Affiliation can not be empty

Please enter a valid email address.

Your Email can not be empty

The content can not be empty

The option can not be empty