녹아웃(KO) 마우스 | Cyagen Korea

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Strain Background: C57BL/6N

 

Construction Strategy:

The humanized LIGHT mouse model (hLIGHT) is developed by knockin at the mouse LIGHT locus, and expresses a chimeric LIGHT with a human extracellular, human transmembrane and murine intracellular domain.

 

hLIGHT Features:

  •   hLIGHT expression displays a physiological regulation and expression pattern
  •   Fully functional mouse immune system
  •   Lack of expression of the murine target gene, thus avoiding cross-reactivity

 

Research and Application:

The hLIGHT mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint (ICP) VISTA in fully immunocompetent mice.

 

Validation Data:

Figure 1. hLIGHT is expressed on activated splenic T cells. Expression of human LIGHT (hLIGHT) on splenic Teff and CD8 T cells activated with αCD3/αCD28 in presence of rmIL-2 for 24h. hLIGHT is expressed on spleen effector T cells and CD8 T cells, but not on splenic T cells of WT mice.

Figure 2. hLIGHT stimulation in hLIGHT models increases T cell activation. T cells from wild-type and hLIGHT mice were stained with CTV and activated with coated amCD3, with or without recombinant mouse (rm) or human LIGHT, and with or without a-human LIGHT. At day 4 proliferation was measured by FACS (A), and IL-2 production was measured in the supernatant (B).

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