녹아웃(KO) 마우스 | Cyagen Korea

Citations

What Our Customers Say

“Among several commercial services who have claimed to be specialized to generate mouse models with genetic modifications, I recognize that Cyagen is one of the best. We are looking forward to having more cooperation with them.”

Korea Brain Research Institute (KBRI)

more ›

Contact Us

영업일 기준 1-2일 내에 답변해 드리겠습니다.

*

The username is required

*

The user's email is required

Please enter a valid email address.

*

The user's phone is required

Please enter a valid phone address.

*

The content is required

*

 

 

Strain Background: C57BL/6N

 

Construction Strategy:

The design of the CTLA-4 humanized model, is developed by knockin at the mouse CTLA-4 locus, enables the expression of: a) the full length chimeric CTLA-4 isoform (human extracellular domain and mouse intracellular domain); and b) the soluble isoform (human extracellular domain).

 

hCTLA-4 Features:

  •   The PD-1 extracellular domain is entirely humanized
  •   hCTLA-4 expression displays physiological regulation and expression pattern
  •   The domain of mouse CTLA-4 protein is retained to ensure normal intracellular signal transduction.
  •   Fully functional mouse immune system
  •   Lack of expression of the murine target gene, thus avoiding cross-reactivity

 

Research and Application:

The hCTLA-4 mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint (ICP) CTLA-4 in fully immunocompetent mice. And you can also entrust Cyagen to construct CTLA-4 conditional knockout mice for a tissue-specific assessment of drug specificity and toxicity.

 

Validation Data:

Figure 1. Immune cell (T cells, B cells, NK cells, DC cells and monocytes) distribution in hCTLA-4 mice is similar to that of WT mice, and immune cells of mice function normally.

Figure 2. hCTLA-4 is functional: α-human CTLA-4 decrease hCTLA-4 Tregs suppressive activity. Isolated CD4+CD25- (Teff) labeled with CFSE and activated with soluble αmCD3 and APC +/- (CD4+CD25+) Treg and CTLA-4 antibody. (A) CFSE dilution was assessed by flow cytometry; (B) IL-2 production was measured by ELISA in the supernatant.

Figure 3. hCTLA-4 expression pattern in hCTLA-4 mice recapitulates mCTLA-4 in WT mice. hCTLA-4 and mCTLA-4 expression on αCD3- activated splenocytes on (A) Tregs (viable, CD3+CD4+CD25+FoxP3+), (B) conventional CD4+ (viable, CD3+CD4+FoxP3-) at day 3, and (C) CD8+ (viable, CD3+CD8+) T cells at indicated time points.

Figure 4. In vivo anti-tumor activity is observed in response to anti-human CTLA-4 in hCTLA-4 mice.

위에 제품이나 서비스에 관심이 있으시면 저희한테 연락 부탁드립니다.

Your First Name can not be empty

Your Last Name can not be empty

Institution/Affiliation can not be empty

Please enter a valid email address.

Your Email can not be empty

The content can not be empty

The option can not be empty