Heterozygous Spink1 c.194+2T>C mutant mice spontaneously develop chronic pancreatitis.
Gut (2019) PMID: 31142585“Among several commercial services who have claimed to be specialized to generate mouse models with genetic modifications, I recognize that Cyagen is one of the best. We are looking forward to having more cooperation with them.”
Korea Brain Research Institute (KBRI)영업일 기준 1-2일 내에 답변해 드리겠습니다.
Strain Background: C57BL/6N
Construction Strategy:
The double-humanized CTLA-4 and LAG3 mouse model (hCTLA-4/hLAG3) was generated by intercrossing hCTLA-4 and hLAG3 mice. Both hCTLA-4 and hLAG3 expressions are regulated by endogenous mouse promoters.
hCTLA-4/hLAG3:
Research & Application:
The hCTLA-4/hLAG3 mouse enables the in vivo efficacy and safety assessment of compounds targeting CTLA-4 and/or LAG3 in fully immunocompetent mice.
Validation Data：
Figure 1. hCTLA-4 expression pattern in hCTLA-4/hLAG3 mice recapitulates mCTLA-4 in wild-type mice. Splenocytes from hCTLA-4/hLAG3 double-homozygous mice were activated with coated αCD3/αCD28 and mIL-2 for 2 days (A, B) or indicated time (C, D). hCTLA-4 and mCTLA-4 expressions were evaluated on A) Tregs (viable, CD3+CD4+CD25+Foxp3+), B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and C, D) CD8+ (viable, CD3+CD8+) T cells.
Figure 2. hLAG3 expression pattern in hCTLA-4/hLAG3 double-humanized model. Splenocytes from wild-type or hCTLA-4/hLAG3 double-homozygous mice were activated with coated αCD3/αCD28 and mIL-2 for 2 days (A, B) or indicated time (C, D). hLAG3 and mLAG3 expression on A) Tregs (viable, CD3+CD4+CD25+Foxp3+), B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and C, D) CD8+ (viable, CD3+CD8+) T cells.