녹아웃(KO) 마우스 | Cyagen Korea

Citations

What Our Customers Say

“Among several commercial services who have claimed to be specialized to generate mouse models with genetic modifications, I recognize that Cyagen is one of the best. We are looking forward to having more cooperation with them.”

Korea Brain Research Institute (KBRI)

more ›

Contact Us

영업일 기준 1-2일 내에 답변해 드리겠습니다.

*

The username is required

*

The user's email is required

Please enter a valid email address.

*

The user's phone is required

Please enter a valid phone address.

*

The content is required

*

 

 

Strain Background: C57BL/6N

 

Construction Strategy:

The double-humanized CTLA-4 and LAG3 mouse model (hCTLA-4/hLAG3) was generated by intercrossing hCTLA-4 and hLAG3 mice. Both hCTLA-4 and hLAG3 expressions are regulated by endogenous mouse promoters.

 

hCTLA-4/hLAG3:

  •   The LAG3 and CTLA-4 extracellular domains are entirely humanized
  •   Physiological regulation and expression pattern of the human LAG3 and CTLA-4
  •   Preservation of the target-ligand interaction
  •   Fully functional mouse immune system
  •   Lack of expression of the murine target gene, thus avoiding cross-reactivity

 

Research & Application:

The hCTLA-4/hLAG3 mouse enables the in vivo efficacy and safety assessment of compounds targeting CTLA-4 and/or LAG3 in fully immunocompetent mice.

 

Validation Data:

Figure 1. hCTLA-4 expression pattern in hCTLA-4/hLAG3 mice recapitulates mCTLA-4 in wild-type mice. Splenocytes from hCTLA-4/hLAG3 double-homozygous mice were activated with coated αCD3/αCD28 and mIL-2 for 2 days (A, B) or indicated time (C, D). hCTLA-4 and mCTLA-4 expressions were evaluated on A) Tregs (viable, CD3+CD4+CD25+Foxp3+), B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and C, D) CD8+ (viable, CD3+CD8+) T cells.

Figure 2. hLAG3 expression pattern in hCTLA-4/hLAG3 double-humanized model. Splenocytes from wild-type or hCTLA-4/hLAG3 double-homozygous mice were activated with coated αCD3/αCD28 and mIL-2 for 2 days (A, B) or indicated time (C, D). hLAG3 and mLAG3 expression on A) Tregs (viable, CD3+CD4+CD25+Foxp3+), B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and C, D) CD8+ (viable, CD3+CD8+) T cells.

위에 제품이나 서비스에 관심이 있으시면 저희한테 연락 부탁드립니다.

Your First Name can not be empty

Your Last Name can not be empty

Institution/Affiliation can not be empty

Please enter a valid email address.

Your Email can not be empty

The content can not be empty

The option can not be empty