녹아웃(KO) 마우스 | Cyagen Korea

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Strain Background: C57BL/6N

 

Construction Strategy:

The humanized CD28 mouse model (hCD28) is developed by Knockin at the mouse CD28 locus, and expresses a chimeric CD28 with a human extracellular, human transmembrane and murine intracellular domain.

 

hCD28 Features:

  •   CD28 expression displays a physiological regulation and expression pattern
  •   Fully functional mouse immune system
  •   Lack of expression of the murine target gene, thus avoiding cross-reactivity

 

Research and Application:

The hCD28 mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint (ICP) CD28 in fully immunocompetent mice.

 

Validation Data:

Figure 1. hCD28 expression pattern in hCD28 mice recapitulates mCD28 in wild-type mice. hCD28 and mCD28 expression on freshly isolated splenocytes on (A) CD8+ (viable, CD3+CD8+) T cells, (B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and (C) Tregs (viable, CD3+CD4+CD25+Foxp3+).

Figure 2. hCD28 functionality: α-human CD28 co-stimulation induces T cell activation and proliferation. Isolated T cells were labeled with CTV and activated with αCD3 and soluble mouse or human αCD28 for 4 days. Representative FACS bar Figures indicating (A) CD25 expression as a marker of activation, and (B) CTV dilution (as a measure of proliferation) gated on live T cells.

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